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| Date | Summary |
|---|
| 03/23 | Verification of linker-switchable PCRs |
| 04/01 | - rePCRs of CD79B, Gal4 and RFP to make linker switchable constructs
- Gel verification of 03/31 and 04/01 PCRs
|
| 04/10 | - rePCRs of CD79A/B, Gal4 and RFP to make linker switchable constructs
- Gel verification of PCRs
|
| 04/11 | redesign CD79A/B primers |
| 04/25 | - rePCRs of CD79A/B, to make linker switchable constructs
- Gel verification of PCRs
|
| 04/30 | - rePCR of CD79B with DMSO
- gel purification of CD79A
- PCR purification of pTet:mRFP and Gal4VP16
- AarI golden gate to make hEF1a:CD79A-pTet:mRFP-Gal4VP16
|