CD79A-12T6-Gal4 4-6, CD79A-12T12 4-5 (6 did not grow in liquid culture)
Digested with ApaI 2 hrs at room temp.
ladder: Hyperladder 1 (shown below)
Seems like #5 of each construct is right, assuming that the 700kb band is too faint to see (which is not an unreasonable assumption) |