Annealed X-TCS and TCS-X half linkers. Diluted to 10 fmol/uL
hEF1a:CD79A-XTCSX-Gal4VP16 Golden Gates
(using new CIDAR protocol https://mv-ezproxy-com.ezproxyberklee.flo.org/wiki/CIDAR_MoClo_Golden_Gate_protocols)
| 3TCS3 | 9TCS3 | 15TCS3 | ||
| 6TCS6 | 9TCS6 | 12TCS6 | ||
| 3TCS9 | 6TCS9 | 9TCS9 | 12TCS9 | 15TCS9 |
| 6TCS12 | 9TCS12 | 12TCS12 | ||
| 3TCS15 | 9TCS15 | 15TCS15 |