Sequencing
Both reads had a silent G=>T point mutation in the last codon of CD79A as planned in the PCR primers. There was also a surprising ~20nt addition to RFP that we may have accidentally amplified but it is an inconsequential insert.
hEF1a: Lyn-TEV Colonies Picked
hEF1a:CD79B-pTet:RFP-Gal4VP16 transformation
hEF1a:CD79A-pTet:RFP-Gal4VP16 Linker Golden gate
Cancelled GG would have failed linkers are ssDNA ultramers
Midiprep and Cryostocks happened without incident