Digest
Plasmid was digested with StuI according to NEB protocol for two hours instead of one
Gel
From Left to right: Hyperladder, hEF1a:CD79A-pTet:RFP-Gal4VP16 sample 1, 2, 3, 4, 5
Expected overlaid bands at 4kb and at slightly over 1.5kb
used NEB two log ladder. Bands appear to be at 4kb and around 1.5kb on all samples. Smearing on sample 3 appears to show contamination in sample three as predicted in the miniprep.