Results
| lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| tube | |||||||||||||||
| enzyme | |||||||||||||||
| result(P/F) | |||||||||||||||
| lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
tube | |||||||||||||||
| enzyme | |||||||||||||||
| result(P/F) | |||||||||||||||
| lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| tube | |||||||||||||||
| enzyme | |||||||||||||||
| result(P/F) | |||||||||||||||
| <insert gel image here> | |||||||||||||||
Plasmids
| Name | pENTR CD79A+STOP | pENTR CD79A-TCS-Gal4VP16 | pENTR Syk-eYFP | pENTR Syk+STOP | pENTR Syk-TEV | pENTR Lyn-TEV | pENTR Lyn+STOP | pENTR Lyn-mKate | Fail state (ggDONR) |
|---|---|---|---|---|---|---|---|---|---|
| virtual gel |  |  |  |  |  |  |  |  |  |
| selected enzyme | AlwNI | BbsI | BbsI | BbsI | BspQI, SapI | BspHI is bad, looks just like failure mode | AlwNI |