- annealed linker oligos
- golden gate into RFP construct
- transformed golden gate product
- all linkers grew colonies except 9TCS6
- picked colonies for all except 9TCS6
- retransformed 9TCS6
- digest and gel verification
- 3TCS3, 3TCS9, 9TCSX show correct band pattern
06/24
- sent 9TCSX for sequencing
hEF1a:CD79A-XnTCSXc-Gal4VP16
3TCS3 | 9TCS3 | 15TCS3 | ||
| midi innoculated | midi verified | midi innoculated | ||
| 6TCS6 | 9TCS6 | 12TCS6 | ||
| midi verified | midi verified | midi verified | ||
| 3TCS9 | 6TCS9 | 9TCS9 | 12TCS9 | 15TCS9 |
| midi verified | to be minied | midi innoculated | to be minied | sequencing |
| 6TCS12 | 9TCS12 | 12TCS12 | ||
| midi verified | to be minied | to be minied | ||
3TCS15 | 9TCS15 | 15TCS15 | ||
| sequencing | to be minied | midi innoculated |