• Created by Unknown User (lylaatta@mit.edu), last modified by Unknown User (clrichar@mit.edu) on Jul 17, 2015 11:31

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06/17

  • annealed linker oligos
  • golden gate into RFP construct 

06/18

  • transformed golden gate product

06/19

  • all linkers grew colonies except 9TCS6 
  • picked colonies for all except 9TCS6
  • retransformed 9TCS6

06/23 

  • digest and gel verification 
  • 3TCS3, 3TCS9, 9TCSX show correct band pattern

06/24

  • sent 9TCSX for sequencing 

hEF1a:CD79A-XnTCSXc-Gal4VP16

3TCS3 9TCS3 15TCS3
     
 6TCS69TCS612TCS6 
     
3TCS96TCS99TCS912TCS915TCS9
     
 6TCS129TCS1212TCS12 
     
3TCS15 9TCS15 15TCS15
     
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