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hEF1a:CD79A-XnTCSXc-Gal4VP16

3TCS3 9TCS3 15TCS3
     
 6TCS69TCS612TCS6 
  no colonies  
3TCS96TCS99TCS912TCS915TCS9
     
 6TCS129TCS1212TCS12 
     
3TCS15 9TCS15 15TCS15
     
All linkers9TCS6

annealed linker oligos

golden gate 06/17

 
transformed 06/18 
pick colonies 06/19retransformed 06/19
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