Sequencing
Both reads had a silent G=>T point mutation in the last codon of CD79A as planned in the PCR primers. There was also a surprising ~20nt addition to RFP that we may have accidentally amplified but it is an inconsequential insert.
Sequencing
Both reads had a silent G=>T point mutation in the last codon of CD79A as planned in the PCR primers. There was also a surprising ~20nt addition to RFP that we may have accidentally amplified but it is an inconsequential insert.