PCR of CD79A
Gel shows successful PCR of CD79A+stop from pENTR CD79A (pDONR223).
Forward primer: iGEM KRB 003 F
Reverse primer: iGEM KRB 004 R
Annealing temperature: 60C
Run using samples before PCR purification.
PCR purified 23 June 2014.
Restriction Digest Verification of pENTR
1 July 2014:
See gel here
| Correct plasmids | Failed plasmids |
|---|---|
| CD79A+stop (4) | CD79A+stop (1) CD79A+stop (2) CD79A+stop (3) |