PCR of CD79A

Gel shows successful PCR of CD79A+stop from pENTR CD79A (pDONR223).

Forward primer:  iGEM KRB 003 F

Reverse primer:  iGEM KRB 004 R

Annealing temperature:  60C

Run using samples before PCR purification.

PCR purified 23 June 2014.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here

Correct plasmidsFailed plasmids
CD79A+stop (4)

CD79A+stop (1)

CD79A+stop (2)

CD79A+stop (3)

CD79A+stop (5)

 

 

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