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titleName Conventions

For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

...

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 
waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

miRNA GroupTreatment Group Group
NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYOTHERNOTES
Antibody Group
pEXPR Gal4UAS:eYFPhEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

Antibodiesdesignedin someone else's freezerexpression      
pEXPR Gal4UAS:mKatehEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      
pEXPR Tre-TEVphEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      Hef1a-rtTa
pEXPR hEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      CD79A WTpossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock  
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

pENTR hEF1a

Antibodiesdesigned expression CD79B WTminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock     
pEXPR TRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

Antibodiesdesigned expressionCD79A-TCS-Gal4VP16planned       CD79B-TCS-Gal4VP16
pEXPR TRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

Antibodiesdesigned expressionplanned      
pEXPR PTet: Syk-TEVp 

pENTR Syk-TEVp

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: eBFPpENTR eBFPAntibodiesin storage?expression  Lyn WTminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock    
pEXPR Gal4/UAS: mKate-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labexpressionLyn-eYFPplanned       Lyn point mutated (always phosphorylate)
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentry researching  IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry   

Syk WT

IDT miniprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock
pENTR CD79A (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU   
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentrySyk-eYFP (mutant non phosphorylate)planned       
pENTR CD79B (pDONR223)Syk-TEVp-Antibodiesin storageentryiGEM -20planned  DNASU  
pENTR CD79B+stoppENTR CD79B (pDONR223)Antibodiesin constructionentry   GMab k on order  
pENTR CD79A-TCS-Gal4VP16pENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentry      
pENTR CD79B-TCS-Gal4VP16

pENTR CD79B
pENTR Gal4VP16

Antibodiesin constructionentryGMab Hon order       
pENTR Lyn (pDONR223)GMabH-TCS-Gal4VP16-Antibodiesin storageentryiGEM -20  addgeneplanned  
pENTR Lyn+stoppENTR Lyn (pDONR223)Antibodiesin constructionentry      
pENTR Lyn-mKatepENTR Lyn
pENTR mKate		Antibodiesin constructionentryhsa-miR-181cplanned       
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Syk+stoppENTR Syk (pDONR223)Antibodiesin constructionentryhsa-miR-9planned       hsa-miR-30d-5pplanned
pENTR Syk-eYFPpENTR Syk
pENTR eYFP		Antibodiesin constructionentry       hsa-miR-144-5p
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		Antibodiesin constructionentryplanned       
pENTR eBFPhsa-let-7f-5p-Antibodiesin storage?entryplanned       has-miR-125b
pENTR L1_mKate_L2-Antibodiesin storageentryplanned      
pENTR rtTA-Antibodiesin storageentry  has-miR-146a  planned  
pENTR_L1_EYFP_L2-Antibodiesin storageentry      
pEXPR TRE-t:BACE2-eYFPhEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlockplanned    IDT  
gBlock mIgM Gmab Light-AntibodiesorderedgBlock   IDT  
pEXPR Tre: BACE2-YFP
  • pENTR
    • L4--TRE-t--R1
in the lab already
  • L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpression       

pENTR L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionentry  planned    

Q1-BACE2-Q2

BACE2Treatmentin constructionPCR product      
BACE2n/aTreatmentin the labexpression      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR product  in -80plasmid AL    
pEXPR eYFPn/aTreatmentin the lab alreadyexpression      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR Hef1a:BACE1-eYFPplannedTre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned expression       

pENTR

L4--Hef1a--R1		in the lab already

L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry       
pENTR L1

Q1-BACE1-

BACE1-eYFP--L2

Q2

BACE1Treatmentplanned PCR product      
BACE1n/aTreatmentin the labexpressionto be ordered       

Q2-eYFP-QX

pEXPR eYFPTreatmentin the lab alreadyPCR product      

pENTR Tre

n/aTreatmentin the labentry       

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR U6:mKate-miRNA(intronic)planned Treatmentin planningexpression       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) Treatmentin planningexpressionplanned      

ggDONR L1_LacZ_L2

 Receptorin storageplasmid+4 in iGEM roomLA- KyleExperiment 1    

 

 

 

ggDONR L1_LacZ_L2 (New)

 
  • ggDONR
Receptorin storagewere not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enoughplasmid+4 in iGEM roomLA- KyleBrandon  
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title...
ggDONR New

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

plasmid+4 in iGEM roomLA-

pENTR L4_Hef1a_R1

 Receptorin labplasmid   Brandon  
  • Hef1a Entry Vector
in planning -
Expand
title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
  • Destination Vector
in planning -
Expand
 
title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorwaiting on partplasmid      LirB2 Expression Vectorin construction - waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 gBlockReceptorto be verifiedplasmid  -4 in iGEM roomSS-   
    • LilrB2 Entry Vector
in construction -
Expand
 
title...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

plasmid

LilrB2 gBlock

      Receptorin
      • LilrB2 gBlock
used in Golden Gate - in storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB_L2

 

pENTR L4_Hef1a_R1

ReceptorWaiting on partexpression     
  • PirB Expression Vector
in construction - waiting on entry vector

pENTR L1_PirB_L2

PirB gBlock C terminus

PirB gBlock N terminus

Receptorto be verifiedentry      in construction -
Expand
 
    • PirB Entry Vector
title...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

       Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry
      • PirB gBlock C terminus
used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT  
      • PirB gBlock N terminus
used in Golden Gate - in storage RRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentrygBlock-20 in iGEM roomLA-IDT  
         
RRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh