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For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

NAMESTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYOTHER
Antibody Group
pEXPR Gal4UAS:eYFPin someone else's freezerexpression      
pEXPR Gal4UAS:mKatein someone else's freezer

expression

      
pEXPR Tre-TEVpin someone else's freezerexpression      
Hef1a-rtTain someone else's freezerexpression      
CD79A WTpossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock     
CD79B WTminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock     
CD79A-TCS-Gal4VP16planned       
CD79B-TCS-Gal4VP16planned       
Lyn WTminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock     
Lyn-eYFPplanned       
Lyn point mutated (always phosphorylate)researching       

Syk WT

miniprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock     
Syk-eYFP (mutant non phosphorylate)planned       
Syk-TEVpplanned       
GMab kon order       
GMab Hon order       
GMabH-TCS-Gal4VP16planned       
miRNA Group
hsa-miR-181cplanned       
hsa-miR-9planned       
hsa-miR-30d-5pplanned       
hsa-miR-144-5pplanned       
hsa-let-7f-5pplanned       
has-miR-125bplanned       
has-miR-146aplanned       
Treatment Group
pEXPR TRE-t:BACE2-eYFPplanned       
    • pENTR L4--TRE-t--R1
in the lab already       
    • pENTR L1--BACE2-eYFP--L2
planned       
in -80plasmid AL    
      • eYFP
in the lab already       
    • pDEST R4--R2
in the lab already       
pEXPR Hef1a:BACE1-eYFPplanned       
    • pENTR L4--Hef1a--R1
in the lab already       
    • pENTR L1--BACE1-eYFP--L2
planned       
      • BACE1
to be ordered       
      • eYFP
in the lab already       
    • pDEST R4--R2
in the lab already       
pEXPR U6:mKate-miRNA(intronic)planned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)planned       
Receptor Group
Experiment 1        
  • ggDONR
were not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enoughplasmid+4 in iGEM roomLA- Kyle  
  • ggDONR New
will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stockplasmid+4 in iGEM roomLA-Brandon  
  • Hef1a Entry Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • Destination Vector
in planning - will be used to make LilrB2 expression vectorplasmid      
  • LirB2 Expression Vector
in construction - waiting on entry vectorplasmid      
    • LilrB2 Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNAplasmid      
      • LilrB2 gBlock
used in Golden Gate - in storagegBlock-20 in iGEM roomLA- IDT  
  • PirB Expression Vector
in construction - waiting on entry vector       
    • PirB Entry Vector
in construction - failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA       
      • PirB gBlock C terminus
used in Golden Gate - in storagegBlock-20 in iGEM roomLA-IDT  
      • PirB gBlock N terminus
used in Golden Gate - in storage gBlock-20 in iGEM roomLA-IDT  
         
         
         

 

 

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