changes.mady.by.user Unknown User (clrichar@mit.edu)
Saved on Aug 08, 2014 17:05
changes.mady.by.user Unknown User (asjm@mit.edu)
Saved on Aug 12, 2014 10:56
Tests the effectiveness of a Syk-TEV cofactor in the tango system with no transfected Lyn, only possible endogenous Lyn.
Capstone Experiment to test Syk-TEV without Lyn.
hEF1a: Gmab H
hEF1a: Gmab L
hEF1a: CD79A
hEF1a: CD79B
TRE: Syk-TEV
hEF1a: rtTA
UAS: mKate
Well 1
HEK293
Well 2
Well 3
Well 4
Well 5
Well 6
Well 7
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We expect the tango cleavage system to have virtually no difference between active and inactive receptors because the BCR requires Lyn to recruit Syk.
Day1
Seed the cells on a plastic plate using splitting cells and seeding plates protocol
Day2
Transfect the cells using either the lipo2k protocol
Day3
add a dox ladder
Day4
do standard cytometry prep
