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Tests the effectiveness of a Syk-TEV cofactor in the tango system with no transfected Lyn, only possible endogenous Lyn.

Capstone Experiment to test Syk-TEV without Lyn.

hEF1a: Gmab H

hEF1a: Gmab L

hEF1a: CD79A

hEF1a: CD79B

TRE: Syk-TEV

hEF1a: rtTA

UAS: mKate

Well 1

HEK293

Well 2

HEK293

Well 3

HEK293

Well 4

HEK293

Well 5 

HEK293

Well 6 

HEK293

Well 7

HEK293

Well 8 

HEK293

Well 9 

HEK293

Well 10 

HEK293

Well 11 

HEK293

Well 12 

HEK293

Well 13 

HEK293

Well 14 

HEK293

Well 15 

HEK293

Well 16 

HEK293

Well 17 

HEK293

Well 18 

HEK293

Well 19 

HEK293

Well 20

HEK293

Well 21 

HEK293

Well 22 

HEK293

Well 23 

HEK293

Well 24 

HEK293

We expect the tango cleavage system to have virtually no difference between active and inactive receptors because the BCR requires(question) Lyn to recruit Syk.

Day1

Seed the cells on a plastic plate using splitting cells and seeding plates protocol

Day2

Transfect the cells using either the lipo2k protocol

Day3

add a dox ladder

Day4

do standard cytometry prep

 

 

 

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