You are viewing an old version of this page. View the current version.
Compare with Current View Page History
« Previous Version 5 Next »
Tests the effectiveness of a Syk-TEV cofactor in the tango system with no transfected Lyn, only possible endogenous Lyn.
Capstone Experiment to test Syk-TEV without Lyn.
hEF1a: Gmab H
hEF1a: Gmab L
hEF1a: CD79A
hEF1a: CD79B
TRE: Syk-TEV
hEF1a: rtTA
UAS: mKate
Well 1
HEK293
Well 2
Well 3
Well 4
Well 5
Well 6
Well 7
Well 8
Well 9
Well 10
Well 11
Well 12
Well 13
Well 14
Well 15
Well 16
Well 17
Well 18
Well 19
Well 20
Well 21
Well 22
Well 23
Well 24
We expect the tango cleavage system to have virtually no difference between active and inactive receptors because the BCR requires Lyn to recruit Syk.
Day1
Seed the cells on a plastic plate using splitting cells and seeding plates protocol
Day2
Transfect the cells using either the lipo2k protocol
Day3
add a dox ladder
Day4
do standard cytometry prep