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DpnI Digest

Ran DpnI restriction digest on products from PCR of TEVp (with 15aa linker), essentially following protocol from 2014 June 24.

Conditions for DpnI digest:

Chemical
15aa-TEVp
Starting PCR reaction24 uL
DpnI1.7 uL
NEBuffer 2.13.4 uL
dH2O5.9 uL
TOTAL35 uL

Incubated at 37C for 45 minutes.  Heat shock at 80C for 20 minutes.

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