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To do:
  • High school presentations
  • Re-culture Syk and Lyn
  • Update our section of the parts list
  • Design Lyn-TEVp fusion

Notes:

  • Syk and Lyn concentrations found to be only ~15 ng/uL, so need to grow new cultures and re-miniprep
  • Primers iGEM2014-EE-[003,006] found to be missing spacer before BsaI site; corrected primers re-ordered
To do:
  • Miniprep Syk/Lyn and nanodrop
  • Sequence Syk/Lyn
  • Discuss antibody orders with Brian
  • NEGEM
To do:
  • All the PCRs!
    • CD79A + stop codon
    • CD79A + TCS
    • mKate (for fusion proteins)
  • Sequence Syk/Lyn
To do:
  • Image gel from June 22
  • Golden Gate PCRs from June 22
  • More PCRs with new primers

To do:

  • Redo Syk-eYFP PCR
  • Image gel for yesterday's PCRs
  • Purify and gel Syk-eYFP PCR
  • Run remaining PCRs
  • Purify and gel remaining PCRs
  • Golden Gates
  • Put gel images on parts pages
  • Update wiki

Notes:

  • All primers arrived except for CR006/007, so we can do all the PCRs except for Lyn.

 

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