| TASK | WhoDunnit | Observed |
|---|---|---|
| Split cells | AG, LA, SS | |
| Seed plates | AG, LA, SS | |
| Transfection | AG | SS, LA |
| Imaging (FloCyto) |
Practice Transfection:
CONTROLS:
- -ve/-ve: no actions performed on cells
- -ve: perform protocol, excluding DNA, or with dummy DNA
- +ve: tri-color control (perform experiments with YFP, BFP, mKATE separately, and one with all 3: allows for comparison of color/intensity when imaging)
- Check for cell death
- Measurement