Parts List

Name Conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status Words

Word	Meaning
in planning	being designed in geneious
designed	plasmid design completed in geneious
in lab	someone else in the lab has it
ordered
in construction
constructed
in storage	we have it in our fridge/freezer, etc

NAME	Dependency	GROUP	STATUS	TYPE	LOCATION	INITIALS	CONSTRUCTED ON	OBTAINED FROM	PRIORITY	NOTES
pEXPR hEF1a: Gmab Heavy	pENTR Gmab Heavy pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: Gmab Light	pENTR Gmab Light pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: CD79A	pENTR CD79A pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: CD79B	pENTR CD79B pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: CD79A-TCS-Gal4VP16	pENTR CD79A-TCS-Gal4VP16 pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: CD79B-TCS-Gal4VP16	pENTR CD79B-TCS-Gal4VP16 pENTR hEF1a	Antibodies	designed	expression
pEXPR hEF1a: Lyn	pENTR Lyn pENTR hEF1a	Antibodies	designed	expression
pEXPR PTet: Lyn-mKate	pENTR Lyn-mKate pENTR PTet	Antibodies	designed	expression
pEXPR PTet: Syk-eYFP	pENTR Syk-eYFP pENTR PTet	Antibodies	designed	expression
pEXPR PTet: Syk-TEVp	pENTR Syk-TEVp pENTR PTet	Antibodies	designed	expression
pEXPR PTet: eBFP	pENTR eBFP	Antibodies	in storage?	expression
pEXPR Gal4/UAS: mKate	-	Antibodies	in storage	expression	iGEM -20	CR	N/A	Velia	High
pEXPR hEF1a: rtTA	pENTR rtTA	Antibodies	in storage	expression	iGEM -20	CR	N/A	Velia	High
pEXPR Gal4UAS:eYFP	-	Antibodies	in storage	expression	iGEM -20	CR	N/A	Velia	High
pEXPR Tre-TEVp	-	Antibodies	in lab	expression
pENTR Gmab Heavy	gBlock mIgM Gmab Heavy	Antibodies	Ordered	entry				IDT
pENTR Gmab Light	gBlock mIgM Gmab Light	Antibodies	Ordered	entry				IDT
pENTR CD79A (pDONR223)	-	Antibodies	in storage	entry	iGEM -20			DNASU
pENTR CD79A+stop	pENTR CD79A (pDONR223)	Antibodies	in construction	entry
pENTR CD79B (pDONR223)	-	Antibodies	in storage	entry	iGEM -20			DNASU
pENTR CD79B+stop	pENTR CD79B (pDONR223)	Antibodies	designed	entry
pENTR CD79A-TCS-Gal4VP16	pENTR CD79A pENTR Gal4VP16	Antibodies	in construction	entry
pENTR CD79B-TCS-Gal4VP16	pENTR CD79B pENTR Gal4VP16	Antibodies	designed	entry
pENTR Lyn (pDONR223)	-	Antibodies	in storage	entry	iGEM -20			addgene
pENTR Lyn+stop	pENTR Lyn (pDONR223)	Antibodies	designed	entry
pENTR Lyn-mKate	pENTR Lyn pENTR mKate	Antibodies	in construction	entry
pENTR Syk (pDONR223)	-	Antibodies	in storage	entry	iGEM -20			addgene
pENTR Syk+stop	pENTR Syk (pDONR223)	Antibodies	designed	entry
pENTR Syk-eYFP	pENTR Syk pENTR eYFP	Antibodies	designed	entry
pENTR Syk-TEVp	pENTR Syk pEXPR Tre-TEVp	Antibodies	designed	entry
pENTR eBFP	-	Antibodies	in storage?	entry
pENTR mKate	-	Antibodies	in storage	entry
pENTR rtTA	-	Antibodies	in storage	entry
pENTR eYFP	-	Antibodies	designed	entry
pENTR Gal4VP16	-	Antibodies	designed	entry
gBlock mIgM Gmab Heavy	-	Antibodies	ordered	gBlock				IDT
gBlock mIgM Gmab Light	-	Antibodies	ordered	gBlock				IDT

pEXPR Tre: BACE2-YFP		Treatment	in the lab
pENTR L1--BACE2-eYFP--L2		Treatment	in construction
BACE2		Treatment	in construction
eYFP		Treatment	in the lab
pENTR Tre		Treatment	in the lab
pDEST R4--R2		Treatment	in the lab
pEXPR U6:mKate-miRNA(intronic)		Treatment	planned
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)		Treatment	planned
Experiment 1
ggDONR L1-LacZ-L2		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Kyle		... not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
ggDONR L1-LacZ-L2 (New)		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Brandon		... will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
pENTR L4-Hef1a-R1		Receptor	in lab	plasmid						... will be used to make LilrB2 expression vector
pDEST		Receptor	in lab	plasmid						... will be used to make LilrB2 expression vector
pEXPR Hef1a:LilrB2		Receptor	in construction	plasmid						waiting on entry vector
pENTR L1-LilrB2-L2		Receptor	in construction	plasmid						... failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
LilrB2 gBlock		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
pEXPR Hef1a:PirB		Receptor	in construction							waiting on entry vector
pENTR L1-PirB-L2		Receptor	in construction							... failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
PirB gBlock C terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB gBlock N terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PDONR MAV1212	MAV1212	miRNA sensing	in storage	donor	-20 in iGEM room	RR	N/A	Jeremy Gam	High	Original Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2	-	miRNA sensing	in lab?	entry	?	RR	N/A	N/A	High	Stock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4	-	miRNA sensing	in storage	entry	-20 in iGEM room	RR	N/A	N/A	High
pEXPR hEF1A:l7ae	MAV1212	miRNA sensing	designed	expression	N/A	RR	N/A	N/A	High
pENTR R1_mKate_L4	-	miRNA sensing	in storage	entry	-20 in iGEM room	RR	N/A	N/A	High
MAV1212 hEF1A:eBFP2	MAV1212	miRNA sensing	in storage	positive control plasmid	-20 in iGEM room	RR	N/A	Jeremy Gam	Medium
PENTR R1_eYFP_L4	-	miRNA sensing	in storage	entry	-20 in iGEM room	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:144	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:181c	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:mKate2:144:181c	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:30d	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:let7f	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:125b	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:146a	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
pEXPR MAV1212 hEF1A:eYFP:30d:let7f:125b:146a	MAV1212	miRNA sensing	in planning	expression	N/A	RR	N/A	N/A	High
Q_144_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High
Q_181c_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High
Q_30d_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High
Q_let7f_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High
Q_125b_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High
Q_146a_Q	miRNA target site	miRNA sensing	in lab	target site	?	RR	N/A	IDT	High

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