| TASK | WhoDunnit |
|---|---|
| Split cells | AG, LA, SS |
| Seed plates | AG, LA, SS |
| Transfection | |
| Imaging (FloCyto) |
Practice Transfection:
CONTROLS:
- -ve/-ve: no actions performed on cells
- -ve: perform protocol, excluding DNA
- +ve: tri-color control (perform experiments with YFP, BFP, mKATE separately, and one with all 3: allows for comparison of color/intensity when imaging)