Description:
Making HEK293 expressing LilrB2 and PirB on the cell membrane. Will test binding to receptors using the beta-amyloid oligomers and monomers in Experiment 4.
Plan:
- Order LilrB2 and PirB gBlocks
- Simulate Golden Gate on Geneious and make sure it works
- Golden Gate gBlocks into ggDONR
- Transform bacteria with Golden Gate product and plate
- Pick colonies and grow in liquid culture
- Miniprep cells
- LR into pDEST