Parts List

For priority: 1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status

Word	Meaning
in planning	being designed in geneious
designed	plasmid design completed in geneious
in lab	someone else in the lab has it
ordered
in construction
constructed
in storage	we have it in our fridge/freezer, etc

NAME	Dependency	GROUP	STATUS	TYPE	LOCATION	INITIALS	CONSTRUCTED ON	OBTAINED FROM	PRIORITY	NOTES
pEXPR hEF1a: Gmab Heavy	pENTR Gmab Heavy	Antibodies	designed	expression
pEXPR hEF1a: Gmab Light		Antibodies	designed	expression
pEXPR hEF1a: CD79A		Antibodies	designed	expression
pEXPR hEF1a: CD79B		Antibodies	designed	expression
pEXPR hEF1a: CD79A-TCS-Gal4VP16		Antibodies	designed	expression
pEXPR hEF1a: CD79B-TCS-Gal4VP16		Antibodies	designed	expression
pEXPR hEF1a: Lyn		Antibodies	designed	expression
pEXPR PTet: Lyn-mKate		Antibodies	designed	expression
pEXPR PTet: Syk-eYFP		Antibodies	designed	expression
pEXPR PTet: Syk-TEV		Antibodies	designed	expression
pEXPR PTet: eBFP		Antibodies	In Freezer	expression
pEXPR Gal4/UAS: mKate		Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR hEF1a: rtTA		Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR Gal4UAS:eYFP		Antibodies	in someone else's freezer	expression
pEXPR Tre-TEVp		Antibodies	in someone else's freezer	expression
pENTR Gmab Heavy		Antibodies	Planned	entry
pENTR Gmab Light		Antibodies	Planned	entry
pENTR CD79A (pDONR223)		Antibodies	in freezer	entry
pENTR CD79B (pDONR223)		Antibodies	in freezer	entry
pENTR CD79A-TCS-Gal4VP16		Antibodies	Planned	entry
pENTR CD79B-TCS-Gal4VP16		Antibodies	Planned	entry
pENTR Lyn (pDONR223)		Antibodies	In culture	entry
pENTR Lyn-mKate		Antibodies	Planned	entry
pENTR Syk (pDONR223)	-	Antibodies	in culture	entry
pENTR Syk-eYFP		Antibodies	Planned	entry
pENTR Syk-TEVp		Antibodies	Planned	entry
pENTR eBFP		Antibodies	?	entry
pENTR mKate		Antibodies	?	entry	iGEM -20	CR	N/A	Velia	High
pENTR rtTA		Antibodies	?	entry	iGEM -20	CR	N/A	Velia	High
pENTR eYFP		Antibodies	?	entry
pENTR Gal4VP16		Antibodies	?	entry
gBlock mIgM Gmab Heavy	-	Antibodies	ordered	gBlock						Ordered from IDT
gBlock mIgM Gmab Light	-	Antibodies	ordered	gBlock

pENTR Tre-TEVp		Treatment	in the lab already
pENTR L1--BACE1-eYFP--L2		Treatment	planned
BACE1		Treatment	to be ordered
eYFP		Treatment	in the lab already
pDEST R4--R2		Treatment	in the lab already
pEXPR U6:mKate-miRNA(intronic)		Treatment	planned
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)		Treatment	planned
Experiment 1
ggDONR		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Kyle		not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
ggDONR New		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Brandon		will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
pENTR L4-Hef1a-R1		Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pDEST		Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pEXPR Hef1a:LilrB2		Receptor	in construction	plasmid						waiting on entry vector
LilrB2 Entry Vector		Receptor	in construction	plasmid						ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
LilrB2 gBlock		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB Expression Vector		Receptor	in construction							waiting on entry vector
PirB Entry Vector		Receptor	in construction							failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
PirB gBlock C terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB gBlock N terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PDONR MAV1212	-	miRNA sensing	in storage	donor	-20 in iGEM room	RR	N/A	Jeremy Gam	High	Original Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2	-	miRNA sensing	in lab?	entry	?	RR	N/A	N/A	High	Stock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4	-	miRNA sensing	in storage	entry	-20 in iGEM room	RR	N/A	N/A	High
pEXPR hEF1A:l7ae	MAV1212	miRNA sensing	designed	expression	N/A	RR	N/A	N/A	High
pENTR R1_mKate_L4	-	miRNA sensing	in storage	entry	-20 in iGEM room	RR	N/A	N/A	High

Child pages

Parts List