Parts List

For priority: 1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status

Word	Meaning
in planning	being designed in geneious
designed	plasmid design completed in geneious
in lab	someone else in the lab has it
ordered
in construction
constructed
in storage	we have it in our fridge/freezer, etc
in culture	received in E. coli culture, has not been mini-prepped yet

NAME	Dependency	GROUP	STATUS	TYPE	LOCATION	INITIALS	CONSTRUCTED ON	OBTAINED FROM	PRIORITY	NOTES
pEXPR hEF1a: Gmab Heavy		Antibodies	Planned	expression
pEXPR hEF1a: Gmab Light		Antibodies	Planned	expression
pEXPR hEF1a: CD79A		Antibodies	Planned	expression
pEXPR hEF1a: CD79B		Antibodies	Planned	expression
pEXPR hEF1a: CD79A-TCS-Gal4VP16		Antibodies	Planned	expression
pEXPR hEF1a: CD79B-TCS-Gal4VP16		Antibodies	Planned	expression
pEXPR hEF1a: Lyn		Antibodies	Planned	expression
pEXPR PTet: Lyn-mKate		Antibodies	Planned	expression
pEXPR PTet: Syk-eYFP		Antibodies	Planned	expression
pEXPR PTet: Syk-TEV		Antibodies	Planned	expression
pEXPR PTet: eBFP		Antibodies	In Freezer	expression
pEXPR Gal4/UAS: mKate		Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR hEF1a: rtTA		Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR Gal4UAS:eYFP		Antibodies	in someone else's freezer	expression
pEXPR Gal4UAS:mKate		Antibodies	in someone else's freezer	expression
pEXPR Tre-TEVp		Antibodies	in someone else's freezer	expression
pENTR Gmab Heavy		Antibodies	Ordered	expression
pENTR Gmab Light		Antibodies	Ordered	expression
pENTR CD79A		Antibodies	in stock	expression
pENTR CD79B		Antibodies	in stock	expression
pENTR CD79A-TCS-Gal4VP16		Antibodies	Planned	expression
pENTR CD79B-TCS-Gal4VP16		Antibodies	Planned	expression
pENTR Lyn		Antibodies	In culture	expression
pENTR Lyn-mKate		Antibodies	Planned	expression
pENTR Syk-eYFP		Antibodies	Planned	expression
pENTR Syk-TEV		Antibodies	Planned	expression
pENTR eBFP		Antibodies	?	expression
pENTR mKate		Antibodies	?	expression	iGEM -20	CR	N/A	Velia	High
pENTR rtTA		Antibodies	?	expression	iGEM -20	CR	N/A	Velia	High
pENTR eYFP		Antibodies	?	expression
pENTR mKate		Antibodies	?	expression

gBlock mIgM Gmab Heavy	none	Antibodies	ordered	gBlock
gBlock mIgM Gmab Light	none	Antibodies	ordered	gBlock
pENTR Syk (pDONR223)	none	Antibodies	in culture	entry
pENTR Lyn (pDONR223)	none	Antibodies	in culture	entry
pENTR CD79A (pDONR223)	none	Antibodies	in storage	entry
pENTR CD79B (pDONR223)	none	Antibodies	in storage	entry
pEXPR U6:mKate-miRNA(intronic)		Treatment	planned
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)		Treatment	planned
Experiment 1
ggDONR		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Kyle		not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
ggDONR New		Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Brandon		will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
pENTR L4-Hef1a-R1		Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pDEST		Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pEXPR Hef1a:LilrB2		Receptor	in construction	plasmid						waiting on entry vector
LilrB2 Entry Vector		Receptor	in construction	plasmid						ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
LilrB2 gBlock		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB Expression Vector		Receptor	in construction							waiting on entry vector
PirB Entry Vector		Receptor	in construction							failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
PirB gBlock C terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB gBlock N terminus		Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate

Child pages

Parts List