Parts List

For priority: 1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status

Word	Meaning
in planning	being designed in geneious
designed	plasmid design completed in geneious
in lab	someone else in the lab has it
ordered
in construction
constructed
in storage	we have it in our fridge/freezer, etc

NAME	GROUP	STATUS	TYPE	LOCATION	INITIALS	CONSTRUCTED ON	OBTAINED FROM	PRIORITY	NOTES
pEXPR hEF1a: Gmab Heavy	Antibodies	Planned	expression
pEXPR hEF1a: Gmab Light	Antibodies	Planned	expression
pEXPR hEF1a: CD79A	Antibodies	Planned	expression
pEXPR hEF1a: CD79B	Antibodies	Planned	expression
pEXPR hEF1a: CD79A-TCS-Gal4VP16	Antibodies	Planned	expression
pEXPR hEF1a: CD79B-TCS-Gal4VP16	Antibodies	Planned	expression
pEXPR hEF1a: Lyn	Antibodies	Planned	expression
pEXPR PTet: Lyn-mKate	Antibodies	Planned	expression
pEXPR PTet: Syk-eYFP	Antibodies	Planned	expression
pEXPR PTet: Syk-TEV	Antibodies	Planned	expression
pEXPR PTet: eBFP	Antibodies	In Freezer	expression
pEXPR Gal4/UAS: mKate	Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR hEF1a: rtTA	Antibodies	In Freezer	expression	iGEM -20	CR	N/A	Velia	High
pEXPR Gal4UAS:eYFP	Antibodies	in someone else's freezer	expression
pEXPR Gal4UAS:mKate	Antibodies	in someone else's freezer	expression
pEXPR Tre-TEVp	Antibodies	in someone else's freezer	expression
hsa-miR-181c	miRNA	planned
hsa-miR-9	miRNA	planned
hsa-miR-30d-5p	miRNA	planned
hsa-miR-144-5p	miRNA	planned
hsa-let-7f-5p	miRNA	planned
has-miR-125b	miRNA	planned
has-miR-146a	miRNA	planned
pEXPR TRE-t:BACE2-eYFP	Treatment	planned
pENTR L4--TRE-t--R1	Treatment	in the lab already
pENTR L1--BACE2-eYFP--L2	Treatment	planned
BACE2 (HsCD00076140)	Treatment	in -80	plasmid		AL
eYFP	Treatment	in the lab already
pDEST R4--R2	Treatment	in the lab already
pEXPR Hef1a:BACE1-eYFP	Treatment	planned
pENTR L4--Hef1a--R1	Treatment	in the lab already
pENTR L1--BACE1-eYFP--L2	Treatment	planned
BACE1	Treatment	to be ordered
eYFP	Treatment	in the lab already
pDEST R4--R2	Treatment	in the lab already
pEXPR U6:mKate-miRNA(intronic)	Treatment	planned
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)	Treatment	planned
Experiment 1
ggDONR	Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Kyle		not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
ggDONR New	Receptor	in storage	plasmid	+4 in iGEM room	LA	-	Brandon		will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
pENTR L4-Hef1a-R1	Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pDEST	Receptor	in lab	plasmid						will be used to make LilrB2 expression vector
pEXPR Hef1a:LilrB2	Receptor	in construction	plasmid						waiting on entry vector
LilrB2 Entry Vector	Receptor	in construction	plasmid						ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
LilrB2 gBlock	Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB Expression Vector	Receptor	in construction							waiting on entry vector
PirB Entry Vector	Receptor	in construction							failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
PirB gBlock C terminus	Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate
PirB gBlock N terminus	Receptor	in storage	gBlock	-20 in iGEM room	LA	-	IDT		used in Golden Gate

Child pages

Parts List