| Gene | Promoter | Expression Vector | Why? |
|---|---|---|---|
| GmabH | hEF1a | pZDonor 1-GTW-2 | 1st in Gibson1 |
| GmabL | hEF1a | pZDonor 2-GTW-3 | 2nd in Gibson1 |
| CD79A | hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson1? |
| CD79A-TCS-Gal4VP16 | hEF1a | pZDonor 3-GTW-4 | 3rd in Gibson1? |
| CD79B | hEF1a | pZDonor 4-GTW-5 | 4th in Gibson1? |
| CD79B-TCS-Gal4VP16 | hEF1a | pZDonor 4-GTW-5 | 4th in Gibson1? |
| Lyn | hEF1a | ||
| Lyn-mKate | Tet | ||
| Syk-eYFP | Tet | ||
| Syk-TEVp | Tet | ||
| eBFP | Tet | Control for fluorescence | |
| mKate | GAL4/UAS | Test activation by transcription factor (Gal4VP16) | |
| rtTA | hEF1a | Required for Tet promoters |
Do we want hEF1a - Syk?