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AL - 6/12
added:
1uL of TRE-t (1) @ 5fM
1uL of EYFP @ 5fM
1uL of pDEST 12 @ 10fM
0.5uL of clonase
mixed by pipetting, a few air bubbles in aliquot.
left over night at room temperature
Purpose: practice
JA- 6/12
added:
1uL of Hef1a (1) @ 5fM
1uL of EBFP @ 5fM
1uL of pDEST 12 @ 10fM
0.5uL of clonase
mixed by pipetting, one air bubble in aliquot.
left over night at room temperature
Purpose: practice
JA- 6/13
after both LR reactions (for JA and AL) from 6/12 incubated at room temp for 24 hours, the Transformation protocol was followed exactly
the plated transformed e. coli were left in the 30 C incubator at 1:00pm