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Subgroup Specific:

miRNA: 

  • Contact Jake Beal about construction of miRNA sensors 
  • Look into cell lines 
    • we already have: HEK293, Cho, Hela, ovarian cancer cells, breast cancer cells
    • Ask Samira about what cell lines she is thinking of getting 
  • Get more information about the miRNA 
  • Ensure that the profile is exactly the one you want in preparation for ordering

Treatment:

  • Anaspec contacted about getting a smaller BACE2 assay kit; they said they do not scale down the kit
    • Look into getting kit at a discounted price 
  • Practice LR reaction done in preparation for working with BACE2 
  • Talk to someone about making miRNA for BACE1 inhibition (Samira or Kyle)

Antibody:

  • Gblocks ordered 
  • Ran through experimentation with Brian - experimental procedure "finalized' 
  • More research to be done about family of proteins that appear to be recruited 
  • Look into other sources of Igm so that experimentation can be started earlier 
  • Josh Leonard Northwestern - talk to him about the cell surface receptors he is working on 

Receptor:

  • Put plasmids into parts list

General: 

  • Parts list - look at last year's method of sorting
    • Put plasmids on to that list  

 

 

 

 

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