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  • learn about luciferase assay
miRNAlinkNotes
195http://www.sciencedirect.com.ezproxyberklee.flo.org/science/article/pii/S0361923012001268see 2.4
339-5phttp://www.jbc.org/content/early/2013/12/18/jbc.M113.518241 
107http://www.jneurosci.org/content/28/5/1213.abstract 

 

Questions/concerns about miRNA:

  • "Overall hairpin length and loop size influence the efficiency of Dicer processing"–how should we design the loop?
  • The specificity of base pair matching in the stem influences processing efficiency
  • Which of the two strands from the stem is incorporated into the RNA-induced silencing complex?
  • miRNA's are readily degraded by RNase (present ubiquitously in cells). So we should be sure to inhibit RNase when working with miRNA's; this inhibitor is available for purchase or might already be in the lab.

Pay attention to cross talk. look for foreign miRNA

BLAST NCIB

 

 

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