Subgroup Specific
Receptor Group:
- Look into making a plasmid table organizing all the plasmids that we will be using
miRNA Group:
- Look into contacting and setting up a meeting with Jake Beal
- 12 miRNA may be a little too much - look into constructing sensors for individual up-regulated or down-regulated miRNA and then look into combining two or more of these sensors
- Contact Jeremy Gam about his miRNA sensing circuit
- start looking into getting the miRNA in order to start testing
- What is the test bed?
- Cell that doesn't have any of the miRNA and add the siRNA for the up-regulated miRNA
- Mimicking the down-regulated "phenotype" will be harder
- Alzheimer's disease model - look into that
- Journal Club on the miRNA profile paper
Treatment Group:
- What we are currently doing:
- Looking into directly assaying BACE2 activity - kit available
- look into getting a smaller kit
- Fusing together BACE2 with YFP
- Looking into miRNA that inhibit BACE1 expression
- Practice transfection with a constitutive promoter and an inducible
- Looking into directly assaying BACE2 activity - kit available
- BACE1 has a Bsa1 site in the middle of it - look into how that would affect cloning strategy
- Look into potential cross-talk resulting from the miRNA that we are considering using to inhibit BACE1 production
- BLAST: pairwise alignment tool
Antibody Group:
- "that is a lot of experiments; you should be afraid"
- gBlocks will be ordered today
- Document how the leader sequence was selected on the wiki
Shinjini:
- Specific cytokines that will help microglia go down the phagocytic pathway
- Cytokines are involved in many other processes - might not be realistic to target them for treatment
- Look into using using microglia as vector hosts for our treatment
Beta- amyloid is like having a splinter that you forget to take out... but in your brain