This is a list of experiments that we would like to do:
| What | Why | Parts |
|---|---|---|
| Point mutate Syk to keep it from being phosphorylated | No phosphorylation = no downstream effects | Syk plasmid |
| Point mutate Lyn to make it autophosphorylate | Control: Even if the BCR complex does not otherwise work, we should still see Syk recruitment | Lyn plasmid |
| Check IgM localization using cytometry (see Hombach paper) | IgMkappa IgM heavy CD79a CD79b | |
| Check CD79a/b localization | fluorescently tagged CD79a/b | |
| Check Lyn localization | Lyn-GFP | |
| Check Syk localization | Syk-GFP | |
| Check TEV cleavage | Syk+TEV beta-amyloid CD79-TEVsite-GFP |