You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 4 Next »

  • Purchase BACE2 plasmid (http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140)
  • Design primers for BACE2 and EYFP
  • Run PCR on BACE2 and EYFP
  • Perform golden gate on PCR products and GGDonr
  • Perform LR reaction with products from previous step and backbone
  • Transfect with expression vector from previous step and rtta vector
  • Observe fluorescence (more work needed on details of actual experiment) 
  • No labels