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  1. Purchase BACE2 plasmid (http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140)
  2. Design primers for BACE2 and EYFP
  3. Run PCR on BACE2 and EYFP
  4. Perform golden gate on PCR products and GGDonr
  5. Perform LR reaction with products from previous step and backbone
  6. Transfect with expression vector from previous step and rtta vector
  7. Observe fluorescence (more work needed on details of actual experiment) 
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