Overview
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment | Expected Results | Protocols | Parts | Completed? |
|---|---|---|---|---|
test | lr | gblock1,gblock2 | ||
| golden gate | ||||
| Construct U6/miRNA | ||||
| Assay down-regulation of BACE1 by U6/miRNA | ||||
| Construct miRNA/BACE2 fusion (also including YFP) | ||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 |
Appendix
data sets