Make an entry vectors:
---- L1 ---- LilrB2 ---- L2 ----
---- L1 ---- PirB ---- L2 ----
Make expression vectors:
---- L4 ---- Promoter ---- R1 ----
+ an entry vector = expression vector
Using PCR with entry vectors to make fusion proteins:
Entry vector ---PCR---> Gene + BsaI site + overhang + linker
BFP ---PCR---> BFP + BsaI + overhang
Combine with LacZ entry vector in Golden Gate to make LilrB2-BFP fusion protein or PirB-BFP fusion protein.