3/7/2014
Performed two LR reactions for practice
Aliquot 1:
1 uL of eYFP @ 5 fm/uL
1 uL of TRE-t @ 5fm/uL
1 uL of DEST @ 10 fm/uL
1 uL of clonase
total: 4 uL
Aliquot 2:
1 uL of eYFP @ 5 fm/uL
1 uL of HEF-1a @ 5fm/uL
1 uL of DEST @ 10 fm/uL
1 uL of clonase
let sit at room temperature overnight