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14-Mar-7
LR Reaction:
Label
|
Gene
|
(fmol)
|
Promoter
|
(fmol)
|
Destinatioin
|
(fmol)
|
LR Clonase(uL) |
Total Volume
|
JX 1
|
mKate
|
5
|
Het1a-LacO
|
5
|
1-2
|
10
|
1
|
4
|
JX 2
|
rtTA
|
5
|
Het1a
|
5
|
1-2
|
10
|
1
|
4
|
Reaction Mix |
|
|
|
|
|
|
DNA |
I-Sce-1 |
Buffer |
Water |
Total |
Mass (μg) |
1 |
|
|
|
|
Concentration (ng/μL) |
166 |
|
|
|
|
Volume (μL) |
6.024096 |
1 |
2 |
10.9759 |
20 |
|
|
|
|
|
|
Order |
2 |
4 |
3 |
1 |
|