This recipe will make 10 aliquots of cells for transformation.
1. Grow ~5-6 mL overnight yeast cells in 2xYPAD for >12-16h at 30C 300rpm until cultures have reached saturation.
2. Add 5 mL of overnight cultures into 50 mL of 2xYPAD in a 250 mL bottle. Record the OD600 of the solution (remember to calculate the OD600(corrected) = OD600 of cells - OD600 of 2xYPAD blank). This will likely be around OD600(corrected) ~ 0.1.