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Created by Unknown User (clrichar@mit.edu), last modified by Unknown User (krbrink@mit.edu) on Jul 22, 2014 11:12
Setup
Well 1 Just Cells | Well 2 1000 ug Dummy DNA | Well 3 hEF1a: eBFP 300 ng Dummy DNA 700 ng | Well 4 UAS: eYFP 300 ng hEF1a: eBFP 300 ng Dummy DNA 400 ng | Well 5 hEF1a: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng Dummy DNA 100 ng | Well 6 EMPTY |
Well 7 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 0 nM Dox | Well 8 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 1 nM Dox | Well 9 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 10 nM Dox | Well 10 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 100 nM Dox | Well 11 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 1000 nM Dox | Well 12 hEF1a: rtTA 100 ng TRE: Gal4VP16 300 ng UAS: eYFP 300 ng hEF1a: eBFP 300 ng 2000 nM Dox |
Expected Results
Well 1 No fluorescence | Well 2 No fluorescence | Well 3 Blue Fluorescence | Well 4 Blue Fluorescence Leaky Yellow? | Well 5 Blue Fluorescence Yellow Fluorescence | Well 6 EMPTY |
Well 7 Blue Fluorescence Leaky Yellow | Well 8 Blue Fluorescence Yellow Fluorescence | Well 9 Blue Fluorescence Yellow Fluorescence | Well 10 Blue Fluorescence Yellow Fluorescence | Well 11 Blue Fluorescence Yellow Fluorescence | Well 12 Blue Fluorescence Yellow Fluorescence |