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Thawing 

  1. Pre-warm complete media. 
  2. Place vial of cells in a 37 ºC water bath for 2 minutes until nearly (~ 80%) thawed. Don't leave for too long because DMSO in freezing media makes cells sad. 
  3. Remove the cells from the vial and add slowly into a 15 mL conical tube containing 10 mL pre-warmed Complete Media (DMEM, 10%FBS + NEAA + PenStrepGlut).
  4. Centrifuge for 3 minutes at 1000 × g to pellet cells and remove the supernatant.
  5. Resuspend cells in 10 mL Complete Media, add to a culture dish and put cells in 37C incubator. 
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