Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

STATUS TABLE

Thursday's to do's

• Take B15T9 plate out of incubator, pick colonies
• Midiprep
  • S9TEV
  • B15T15
• Get aliquot of pENTR VP16Gal4 from Jeremy and start LR
• Possibly inoculate midi for B9T9 depending on sequencing results

Wednesday's to do's

• Split cells
• Golden gate/transform B15T9
• Midiprep
  • A15T9
  • B3T15
  • B9T15
  • B12T9
• Inoculate midiprep culture
  • S9TEV
  • B15T15

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

## I'd like to digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)

Non-Lab To Do's 

• Cytometry time 
• Order Cumate 
• Chicken lysozyme, gp120
• Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI