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Created by Unknown User (krbrink@mit.edu), last modified by Unknown User (eersland@mit.edu) on Aug 07, 2015 14:16
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| | | TRE:Syk-6-tTEVp Midi |
|---|
| Enzyme | | EcoRI | EcoRI |
| Tube/Lane | | 1, 2 | 3 |
| Virtual Gel |  | | |
| DNA construct | Constants Definition | TRE:Syk-15-tTEV | TRE:Syk-15-tTEV | TRE:Syk-6-tTEV |
| Tube identifier | | 1 | 2 | MIDI |
| DNA concentration (ng/ul) | | 158.9 | 250.4 | 599.8 |
| Enzyme Name | | EcoRI-hf | EcoRI-hf | EcoRI-hf |
| Buffer Name | | CS | CS | CS |
| strip tube # | | 1/1 | 1/2 | 1/3 |
| water volume | 17.0 | 13.9 | 15.0 | 16.2 |
| DNA volume | | 3.15 | 2.00 | 0.83 |
| buffer volume | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 |
| loading dye | 4 | 4 | 4 | 4 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 |
| gel/lane | #REF! | 1 | 2 | 3 |
| results | | | | |
| notes | | | | |
| virtual gel | | | | |