Digest/Gel 1
| Construct | A-6T9 midi | A-9T9 midi | A-9T15 midi | A-15T3 midi | A-15T15 midi | hEF1a:Gal4 midi |
|---|---|---|---|---|---|---|
| Enzyme | NcoI | NcoI | NcoI | NcoI | NcoI | BsrGI, StuI Cut in hEF1a and Gal4 |
| Tube/Lane | 1 | 2 | 3 | 4 | 5 | 6 |
| Virtual gel |  |  |
| Construct | A-3T15 mini | A-12T9 mini | A-15T9 mini |
|---|---|---|---|
| Enzyme | NcoI | NcoI | NcoI |
| Tube/Lane | 1, 2 | 3, 4 | 5, 6 |
| Virtual gel |