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Monday's To Do's 
  • Oligomerize AB
  • Miniprep, digest, gel
    •  CD79A linker constructs
    • Midis
  • transform IgM-RFP golden gate
  • golden gate TRE:Syk-15-TEVp in gg donr
Tuesday's To Do's
  • Gel for oligo annealing
    • Order new annealing buffer
  • Pick RFP-IgM Colony
  • Syk-15-TEV GG
Saturday's To Do's 
  • Midipreps 
  • CD79A golden gate T4 ligation
  • Transformations
    • CD79A golden gates 
    • IgM RFP golden gate
  • Sequencing
    • 3T3
    • 6T6
    • 6T12
    • 9T3
      Sequencing account information

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Non-Lab To Do's 
  • Cytometry time 
  • Order Cumate 
  • Chicken lysozyme, gp120
  • Antibodies for antigen binding detection
Waiting on primers to do list:
  • TRE:Syk-15-TEVp
  • CD79B-mRFP-G4 PCR
Higher Level To Do's:
  • Decide on other antigen-variable regions we want to test  
  • Is gantenerumab proprietary and can we actually publish with it?

 

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