| TRE:Syk-15-tTEVp | EGSH:mKate | hEf1a:VgEcR-2A-RXR | TRE:mKate | hEF1a:Gal4VP16 | hEF1a:CD79B-TCS-Gal4VP16 | hEF1a:CD79A-3T3-Gal4VP16 | hEF1a:CD79A-6T6-Gal4VP16 | hEF1a:CD79A-6T12-Gal4VP16 | hEF1a:CD79A-9T3-Gal4VP16 | |
|---|---|---|---|---|---|---|---|---|---|---|
| Enzyme | StuI, ApaI Cuts in TRE, Syk, tTEVp and backbone | StuI cuts in mKate and backbone | ApaI Cuts in hEF1a, VgEcR-2A-RxR and bacbone | StuI cuts in TRE, mKate, and backbone | XhoI cuts in hEF1a, Gal4VP16, and backbone | PvuII Cuts in CD79B, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone |
| digest tube/lane | 21,22,23,24 | 1,2,3,4 | 13,14,15,16 | 17,18,19,20 | 5,6,7,8 | 9,10,11,12 | 25 | 26 | 27 | 28 |
| Virtual gel |
Actual failure:
|  |  |  |  |  |  | | | |
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^gap |
