Wednesday's To Do's
- Miniprep, digest, gel:
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:gal4vp16
- TRE:mKate
- hEF1a:CD79B-TCS-Gal4vp16
- Transform golden gates
- CD79A linker golden gates
- TRE:Syk-15-tTEVp
- Innoculate midi cultures:
- hEF1a:tagBFP
- TRE:mKate
- hEF1a:gal4VP16
- EGSH:mKate
- hEF1a:VgEcR-2A-RxR
- hEF1a:CD79B-TCS-Gal4vp16
Tuesday's To Do's
- Sodium Azide solution
- Midi's:
- hEF1a:CymR
- hEF1a-CuO:mKate
- CMV5-CuO:mKate
- TRE:Syk
- Miniprep:
- Golden gate donor mini product
- LRs:
- EGSH:mKate
- hEf1a:VgEcR-2A-RXR
- TRE:mKate
- hEF1a:Gal4VP16 (remember which pDEST)
- hEF1a:CD79B-TCS-Gal4VP16
- hEF1a:tagBFP
- digest:
- hEF1a:CymR
- hEF1a-CuO:mKate
- CMV5-CuO:mKate
- TRE:Syk
- EGSH:mKate
- hEf1a:VgEcR-2A-RXR
- TRE:mKate
- hEF1a:Gal4VP16 (remember which pDEST)
- hEF1a:CD79B-TCS-Gal4VP16
- hEF1a:tagBFP
- Golden Gates
- TRE:Syk-15-tTEVp
- CD79A golden gates
Monday's To Do's
- Oligomerize beta-amyloid
- Non-Lab To Do's:
- Order chicken lysozyme and gp120?
- order antibodies for antigen binding detection
Geneious To Do List:
- Antibody cloning for chicken lysozyme - Kathryn
hef1a sequencing primersBCR 2.0 To Do
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?