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 hEF1a:CymRhEF1a-CuO:mKateTRE:SykEGSH:mKate

hEf1a:VgEcR-2A-RXR

TRE:mKatehEF1a:Gal4VP16hEF1a:CD79B-TCS-Gal4VP16hEF1a:tagBFP
Enzyme

PstI

Cuts in hEF1a, CymR and backbone

StuI

Cuts in hEF1a, mKate and backbone

StuI, ApaI

Cuts in TRE, Syk and backbone

 

ApaI

Cuts in hEF1a, VgEcR-2A-RxR and bacbone

StuI

cuts in TRE, mKate, and backbone

XhoI

cuts in hEF1a, Gal4VP16, and backbone

PvuII

Cuts in CD79B, TCS and backbone

StuI

Cuts in hEF1a, tagBFP and backbone

Virtual gel 
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