hEF1a:eYFP | TRE:eYFP | TRE:mKate | hEF1a:CD79A-RFP-G4 SDM #1 | hEF1a:CD79A-RFP-G4 SDM #2 | hEF1a:CymR | hEF1a-CuO:mKate | CMV5-CuO:mKate | hEF1a:CD79A-3T3-G4 #1 | hEF1a:CD79A-3T9-G4 #1 | hEF1a:CD79A-3T15-G4 #1 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| Enzyme | StuI | BsaI | BsaI | PvuII | PvuII | PvuII | |||||
| Virtual gel | Fail
Success bands | Fail
Success bands | |||||||||
| Concentration (ng/uL) | |||||||||||
| Buffer | |||||||||||
| Enzyme | |||||||||||
| DNA volume | |||||||||||
| H2O volume | |||||||||||
| Buffer volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Enzyme volume | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
Geneious file locations:
| Plasmid | Geneious file location | Failure mode plasmid |
|---|---|---|
| Fluorescent colors | BCR2.0 > Lyla > Misc pEXPRs | pZDonor 1-GTW-2 |