To determine the optimal level of expression of BCR components and Syk-TEVp, we are placing them under the control of inducible/repressible promoters. This experiment aims to characterize the activity of the promoters we are going to use: TREt and pLac.
Approach
We are going to investigate the expression of mKate driven by each of the promoters with varying concentrations of inducer (dox) and repressor (IPTG).